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one particular doable rationalization for your amplified Aβ creation while in the Aph1 L30F/T164A mutant would be that the mutations maximize intricate formation. to check whether the Aph1 L30F/T164A mutants variety a posh with other components, γ-secretase in yeast microsomes was immunoprecipitated with a PS1 antibody towards the loop location (GIL3) (Figure five).
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The position of Aph1 as a scaffold in complex formation is properly-recognised [9,34,35]. as the mutation found in this analyze may have also afflicted advanced formation, we examined the quantity of sophisticated by immunoprecipitation. The quantities of Aph1aL and PS1 fragments (NTF or CTF) within the elaborate were similar during the Aph1aL WT and mutant in yeast and MEF read more cells.
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